Description
The Eagle Biosciences ELISA for IGFBP-2 E05 is a so-called Sandwich-Assay using two specific and high affinity antibodies. The IGFBP-2 in the samples binds to the first antibody coated on the microtiter plate. In the following step the second specific anti-IGFBP-2-Antibody binds in turn to the immobilised IGFBP-2. The second antibody is biotinylated and will be applied in a mixture with a Streptavidin-Peroxidase-Enzyme Conjugate. In the closing substrate reaction the turn of the colour will be catalysed quantitatively depending on the IGFBP-2-level of the samples